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  • br Data Dataset provided in this article

    2018-10-23


    Data Dataset provided in this article represent the results of PPD induction of cassava roots, cultivated in Santa Catarina state, Brazil and spectrophotometric data of enzymatic activities during cassava storage (fresh samples and those stored during 3, 5, 8 and 11 days). Differently from those analysis described in the published manuscript, other analysis were performed such as linear discriminant analysis (LDA) and partial least square discriminant analysis (PLS-DA). Table 1 presents the results of model comparison. LDA model was the best with minor error rate of predicted samples. Fig. 1 shows the variable importance in the first principal analysis (A–D) and principal component analysis (PCA) together with the Eigen values and loadings.
    Experimental design, materials and methods Cassava cultivars were grown in Southern Brazil over the 2011/2012 growing season. Four cultivars were selected for this study, as follows: SCS 253 Sangão (hereinafter SAN), Branco (hereinafter BRA, a landrace), IAC576-70 (hereinafter IAC, a commercial variety), and Oriental (hereinafter ORI, a landrace). On-farm trials were carried out at the Ressacada Experimental Farm (Plant Science Center, Federal University of Santa Catarina, Florianópolis, SC, Brazil-27°35′48″ S, 48°32′57″ W) in September 2011, using the four cassava cultivars noted above. Samples of cassava cuttings for cultivation were provided by the Santa Catarina State Agricultural Research and Rural Extension Agency (EPAGRI) at Urussanga, the official state agriculture agency. The experimental design was in randomized blocks, with 4 blocks (6.3×15m/block) spaced at 1m. Each block consisted of four plots (12×1.2m/plot) spaced at 0.5m. Cassava cuttings (15cm long) were planted upright and spaced at 1×1m. Each plot was considered a treatment, and all crop management was mechanized. Chemical analysis of soil fertility was previously done, and cultivation was performed manually, following agroecological field handlings. Cassava root samples (12 months old) were collected for analysis of non-stored samples and for induction of physiological deterioration under controlled conditions in the laboratory. Immediately after harvest, the CGP 41251 were washed, proximal and distal parts of the root were removed, and cross sections were made (0.5–1cm) over the remaining root, followed by storage at room temperature (66–76% humidity, 25°C). Induction of PPD was performed for 11 days. Monitoring the progress of PPD and associated metabolic disturbances was performed daily after induction of PPD. Non-stored samples and those at 3, 5, 8, and 11 days after PPD induction were collected at each time point, dried (35–40°C) in an oven, milled with a coffee grinder (Model DGC-20N series), and kept for analysis. For enzymatic analysis, fresh samples (batch of seven roots from each cultivar) were collected, grated using a food processor (Walita-Master Plus, Brazil), and stored (−80°C) until analysis. Five independent experiments of PPD were carried out in which a randomized sampling of 3 sliced roots from each plant variety was scored (from 1–10% of PPD to 10–100% of PPD) over the 11-day experimental period. The information was imaged through a digital camera (OLYMPUS FE-4020, 14 megapixel), and the results were analyzed by visual inspection of the images. All enzymatic analysis were done as described in our manuscript (Uarrota et al., 2015).
    Acknowledgements This work was supported by PEC-PG (“Programa de Estudantes Convênio de Pós-Graduação”) coordinated by CAPES (“Coordenação de Aperfeiçoamento de Pessoal de Nível Superior”). CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) has provided a research fellowship to Marcelo Maraschin.
    Data Zingibain (EC 3.4.22.67), is an extensively used protease in food industry for cheese-making or meat tenderization, and therefore its fast and efficient purification is highly required. In the present work, we provide data of the optimal parameters for an efficient purification tool, the Three Phase Partitioning system for high purity and recovery yield of zingibain. A table containing the effect of usual salts and organic solvents on partitioning of zingibain by TPP is given (Table 1). In addition a flow sheet of the workflow employed to purify zingibain from fresh Zingiber officinale Roscoe rhizomes followed by the Kinetics (Michaelis-Menten and Lineweaver-Burk plot) of the purified zingibain are shared (Figs. 1 and 2).